Product Info Summary
SKU: | PB9912 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ZP2 Antibody Picoband™
SKU/Catalog Number
PB9912
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ZP2 Antibody Picoband™ catalog # PB9912. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9912)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ZP2, different from the related mouse sequence by six amino acids, and from the related rat sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9912 is reactive to ZP2 in Human
Applications
PB9912 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
82 kDa
Calculated molecular weight
82.357kDa
Background of ZP2
Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene. The sperm-binding domain on the ZP2 protein is necessary in both humans and mice for oocyte-sperm recognition and penetration of the zona pellucida. It is also responsible for the primary block to polyspermy in mammals. The oocyte has cortical granules peripherally located under the cortex that contain a proteolytic protein called ovastacin. After the sperm binds to ZP2, the cortical granules are exocytosed releasing ovastacin into the perivitelline space. Ovastacin cleaves ZP2 at the N terminus, preventing more sperm from binding and penetrating the oocyte, thus hardening the zona pellucida. Ovastacin is only found in oocytes, and is part of the astacin family of metalloendoproteases. Female mice engineered without ovastacin showed that ZP2 was not cleaved after fertilization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ZP2 using anti-ZP2 antibody (PB9912).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: HEPG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZP2 antigen affinity purified polyclonal antibody (Catalog # PB9912) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZP2 at approximately 82 kDa. The expected band size for ZP2 is at 82 kDa.
Click image to see more details
Figure 2. IHC analysis of ZP2 using anti-ZP2 antibody (PB9912).
ZP2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZP2 Antibody (PB9912) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ZP2 using anti-ZP2 antibody (PB9912).
ZP2 was detected in frozen section of human placenta tissue . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ZP2 Antibody (PB9912) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ZP2 (Source: Uniprot.org, NCBI)
Gene Name
ZP2
Full Name
Zona pellucida sperm-binding protein 2
Weight
82.357kDa
Superfamily
ZP domain family
Alternative Names
zona pellucida glycoprotein 2 (sperm receptor); Zona pellucida glycoprotein 2; zona pellucida glycoprotein ZP2; Zona Pellucida Protein A; zona pellucida sperm-binding protein 2; ZP2; Zp-2; ZPA ZP2 OOMD6, ZPA, Zp-2 zona pellucida glycoprotein 2 zona pellucida sperm-binding protein 2|zona pellucida glycoprotein 2 (sperm receptor)|zona pellucida glycoprotein ZP2|zona pellucida protein A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZP2, check out the ZP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZP2 Antibody Picoband™ (PB9912)
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No publications found for PB9912
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-ZP2 Antibody Picoband™
Question
Will anti-ZP2 antibody PB9912 work on feline WB with cerebellum?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
Our lab technicians have not validated anti-ZP2 antibody PB9912 on feline. You can run a BLAST between feline and the immunogen sequence of anti-ZP2 antibody PB9912 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline cerebellum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-17
Question
We are currently using anti-ZP2 antibody PB9912 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
The anti-ZP2 antibody (PB9912) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-05
Question
Can you help my question with product PB9912, anti-ZP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9912 anti-ZP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-12
Question
Will PB9912 anti-ZP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Patel
Verified customer
Asked: 2019-03-21
Answer
It shows on the product datasheet, PB9912 anti-ZP2 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-21
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary using anti-ZP2 antibody PB9912. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-02
Question
I would like to test anti-ZP2 antibody PB9912 on human ovary for research purposes, then I may be interested in using anti-ZP2 antibody PB9912 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Anderson
Verified customer
Asked: 2015-08-03
Answer
The products we sell, including anti-ZP2 antibody PB9912, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-08-03